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Bacteriostatic Effect of Anogeissus leicarpus Methanolic Leaves Extract

Mohamed ismail garbi, Ahmed Saeed Kabbashi, Ahmed Abdelhafiz Elshikh

Abstract


This study was carried out to investigate the in-vitro antimicrobial activity of A. leiocarpus extract against clinical isolates performed by cup-plate agar diffusion method in five concentrations (100- 6.25 mg/ml) against 3 Gram negative bacteria: Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumonia and 1 Gram positive bacteria: Staphylococcus aureus. The methanolic extract exhibited inhibitory effects against most of the tested microorganisms with zone of inhibition ranging from (21-8 mm). The largest inhibition zone were obtained methanolic extract of A. leiocarpus (leaves) against the Gram negative Escherichia coli(21±0.5mm) in 100 mg/ml concentration, comparison with (32 mm) Gentamicin 40 mgc. and Staphylococcus aureus (11 mm) in 100 mg/ml concentration comparison with (35mm) Gentamicin 40mgc. The methanol extract of A. leiocarpus was considered for further exploration of isolation active compounds analysis.


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