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Investigation of Hepatoprotective Effect of Delonix Regia Leaves in Paracetamol Induced Hepatotoxicity in SD Rats

K M Saiful Islam, Munni Akter, Md. Monir Hossien, Shabnaz Shikder, Sara Benojir Shanta, Md. Imran Hossen, Md. Nahid Hasan


Medicinal plants have been used to treat liver diseases by folk medical practitioners for long time. Delonix regia has been used in traditional medicine to alleviate several diseases. Present study investigated the hepatoprotective effect of Delonix regia leaves in paracetamol induced hepatotoxicity in rats. SD Rats (140–200g) were used. Acute hepatotoxicity was induced by paracetamol (700mg/kg body weight) administered once daily for one week whereas the test extract was given orally throughout the whole experiment at 300 and 600mg/kg body weight. Silymarin (100mg/kg b.w.) was given orally as standard hepatoprotective drug. The degree of hepatoprotection was determined by the estimation of biochemical parameters like SGPT, SGOT, ALP, bilirubin and total protein. The increased levels of hepatic enzymes including SGPT, SGOT, ALP and bilirubin was found in the paracetamol control group, which may be due to the liver cell damage or changes in the cell membrane permeability indicating severity of hepatocellular damage induced by paracetamol. Treatment with Delonix regia and silymarin recovered the increased levels of these hepatic enzymes significantly to reduced levels. The results of the study evidenced that the Delonix regia leaves has shown hepatoprotective activity against paracetamol induced hepatotoxicity in rats.

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Lopez-Virella, MF, Stone PG, Colwell JA (1977), “Serum high density lipoprotein in patients”, Clinical Chemistry, Volume 23, Issue 5, pp. 882−884.

Liu JY, Chen CC, Wang WH, Hsu JD, Yang MY, Wang CY (2006), “The Protective Effect of Hibiscus sabdariffa extract on CCl4 Induced Liver Fibrosis in Rats”, Food Chem Toxicol, Volume 44, Issue 3, pp. 336−343.

Fawcett JK, Scott JE (1960), “A rapid and precise method for the determination of urea”, J Clin Pathol., Volume 13, pp. 156−159.

Deshpande UR, Gadre SG, Raste AS et al. (1998), “Protective effect of turmeric (Curcuma longa L.) extract on carbon tetrachloride-induced liver damage in rats”, Indian J ExpBiol, Volume 36, pp. 573−577.

Farah Hidayah Kamisan, Farhana Yahya, Noor Aisyah Ismail, Syafawati Shamsahal Din, Siti Syariah Mamat, Zalina Zabidi, Wan Noraziemah Wan Zainulddin, Norhafizah Mohtarrudin, Hadijah Husain, Zuraini Ahmad, Zainul Amiruddin Zakaria (2013), “Hepatoprotective activity of methanol extract of melastomamalabathricum leaf in Rats”, J Acupunct Meridian Stud, Volume 6, Issue 1, pp. 52−55.

Don Burke (1 Nov, 2005), “The complete Burke's backyard: the ultimate book of fact sheets”, Murdoch Books, pp. 269, ISBN 978-1-74045-739-2.

Delonix regia, Available from

Stephen H Brown, “Delonix regia. Lee County Extension”, Horticulture Agent, Fort Myers, Florida, Available from

Black M (1980), “Acetaminophen hepatotoxicity”, Gastroenterology, Volume 78, pp. 382−392.

Pacifici GM, Back DJ, Orme ML (1988), “Sulphation and glucuronidation of paracetamol in human liver: assay conditions”, Biochem Pharmacol, Volume 37, pp. 4405−4407.

Jollow DJ, Mitchell JR, Potter WZ, Davis DC, Gillette JR, Brodie BB (1973), “Acetaminophen-induced hepatic necrosis: II. Role of covalent binding in vivo”, J Pharmacol Exp Ther, Volume 187, pp. 195−202.

Potter DW, Hinson JA (1986), “Reactions of N-acetyl-p-benzoquinoneimine with reduced glutathione, acetaminophen and NADPH”, Mol Pharmacol, Volume 30, pp. 33−41.

Hearse DJ, In eds Hearse DJ, De Leiris J, Loisance D, (1979), “Cellular damage during myocardial ischaemia: metabolic changes leading to enzyme leakage”, Enzymes in Cardiology, John Wiley and Sons Ltd., pp. 1−21, New York.

Plaa GL, Zimmerman HJ, In eds. Mc Cuskey RS, Earnest DL (1997), “Evaluation of hepatotoxicity: physiological and biochemical measures of hepatic function”, Comprehensive Toxicology, Cambridge University Press, Volume 9, pp. 97−109, Cambridge, UK.

Drotman RB, Lowhorn GT (1978), “Serum enzymes as indicators of chemical induced liver damage”, Drug Chem Toxicol, Volume 1, pp. 163−171.

Plaa GL, Hewitt WR, In ed. Wallace Hayes A (1989), “Detection and evaluation of chemically in-duced liver injury”, In: Principles and Methods of Toxicology, 2nd ed., Raven Press, New York, pp. 399−428.

Martin P, Friedman LS In eds. Friedman LS, Keeffe EB (1998), “Assessment of liver function and diagnostic studies. In: Handbook of Liver Disease”, Churchill Livingstone, pp. 1−14, Philadelphia.

Alper CA (1974), “Plasma protein measurement as a diagnostic aid”, N. Eng. J. Med., Volume 291, pp. 287−290.

Killingsworth LM (1981), “The Role of high resolution electrophoresis in the clinical evaluation of protein status”, Freehold, Worthington Diagnostics, New Jersey.

Badylak SF, Van Vleet, JF (1981), “Alteration of prothrombin time and activated partial thromboplastin time in dogs with hepatic disease”, Am. J. Vet. Res., Volume 42, pp. 2053−2056.


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