DNA Barcoding: A New Tool for Solving Problems Related To Fungi Identification

Abstract

DNA barcoding is a new term introduced in scientific literatures to identify organisms up to the species level. Nowadays, DNA barcoding has been widely used to identify fungi also. Moreover, DNA barcoding has huge utilities to verify the taxonomic identity of fungi. By using DNA barcoding of nuclear ribosomal internal transcribed spacer (ITS) of the rRNA gene with fungal specific ITS primers, ITS barcodes were generated. After generating they were sequenced and given for BLAST search in GenBank. The homologous sequences of the ITS region of fungi were found out and their relationship is being examined through taxonomic and phylogenetic studies. In the modern era, through sequencing and BLAST search the identification of fungi till species can be done successfully.