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A Review And Recent Update on LCMS

Tajim Makasud Sheikh, Anil Shinde



Liquid Chromatography/Mass spectrum analysis (LC/MS) is quick developing and it’s the popular tool of liquid chromatographers. Liquid chromatography-mass spectrum analysis (LC-MS/MS) could be a technique that uses liquid natural process (or HPLC) with the mass spectrometry. it's AN analytical chemistry technique that mixes the physical separation capabilities of liquid natural process (or HPLC) with the mass analysis capabilities of mass spectrum analysis. (LC-MS/MS) is commonly utilized in laboratories for the qualitative and measurement of drug substances, drug product and biological samples. it's been persistently utilized in drug development at many alternative stages as well as Metabolic Stability Screening, Metabolite Identification moreover as in vivo Drug Screening, Impurity Identification, amide Mapping, Glycoprotein Mapping, Natural product Dereplication, Bio-affinity Screening. LC-MS is now with success applied to routine analysis in several areas, as well as therapeutic drug monitoring (TDM), clinical and rhetorical pharmacological medicine moreover as doping management. This advancement in LCMS was originally and still is oil-fired by the necessity for a lot of powerful analytical and bio-analytical techniques that may accurately and exactly discriminate target analytes from high quality mixtures in a very sensitive and selective means. With recent advancement in instrumentation, the employment of liquid natural process (LC) and mass spectrometry (MS) has become a strong two-dimensional (2D) combined technology.

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Beckett AH and Stenlake GH.2005: Practical Pharmaceutical Chemistry, fourth ed., CBS Publishers and distributors, New Delhi.

Sharma BK.2004: Instrumental methods of chemical analysis, twenty third ed., Goel Publishing House, Meerut.

Arpino, Patrick.1992: Combined liquid chromatography mass spectrometry. Part III. Applications of thermospray”. Mass Spectrometry Reviews, 11: 3. doi:10.1002/mas.1280110103.

Arpino, Patrick.1989: Combined liquid chromatography mass spectrometry. Part I. Coupling by means of a moving belt interface”. Mass Spectrometry Reviews 8: 35. doi:10.1002/mas.1280080103.

Murray, Kermit K. 1997: Coupling matrix assisted laser desorption/ionization to liquid separations”. Mass Spectrometry Reviews 16(5): 283. doi: 10.1002/(SICI)1098 2787(1997)16:5 3.0.CO;2-D.

James J Pitt.2009: Principles and Applications of Liquid Chromatography-Mass Spectrometry in Clinical Biochemistry, Clin Biochem Rev. 30(1): 19–34.

LC-MS: Why use it, and what is it, Metabolite Services at JIC,

He CM1, Cheng ZH1.2013: Chen DF Qualitative and quantitative analysis of flavonoids in Sophora tonkinensis by LC/MS and HPLC. Chin J Nat Med.11 (6): 690-8.

Richard B. van Breemen Liquid chromatography/mass spectrometry of carotenoids Pure 84 Appl. Chem., 69(10): 2061-2066,

Wu, S.-W., et al. 2014 "A novel LC-MS2 product dependent parallel data acquisition function and data analysis workflow for sequencing and identification of intact glycopeptides," Analytical Chemistry, 86: 5478–86.

M.A. Lauber, S.M. Koza, K.J. Fountain. 2013: Waters Application Note 720004571EN Peptide Mapping and Small Protein Separations with Charged Surface Hybrid (CSH) C18 and TFA-Free Mobile Phases.

Shah RP1 , Sahu A, Singh S.2010: Identification and characterization of degradation products of irbesartan using LC-MS/TOF, MS(n), on-line H/D exchange and LC-NMR.J Pharm Biomed Anal. 51(5): 1037-46.

Nair Anroop et. al.2010: / Quantitative Bioanalysis by LC-MS/MS: A Review JPBMS,7(01): 1-9

Sommer.U LC-MS-based method for the qualitative and quantitative analysis of complex lipid mixtures, J Lipid Res. 47(4): 804-14.

Ju-Seop Kang (2012). Principles and Applications of LC-MS/MS for the Quantitative Bioanalysis of Analytes in Various Biological Samples, Tandem Mass Spectrometry - Applications and Principles, Dr Jeevan Prasain (Ed.), ISBN: 978-953-51-0141-3.

Oksman-Caldentey K-M, Inz´e D.2004: Plant cell factories in the post-genomic era: new ways to produce designer secondary metabolites. Trends Plant Sci. 9(9): 433–440.

Lifeng Han, Erwei Liu, Agyemang Kojo, et al. 2015: Qualitative and Quantitative Analysis of Eclipta prostrata L. by LC/MS,” The Scientific World Journal, Article ID 980890, 15 pages

Jin Li “An Improved LC-MS/MS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations,” The Scientific World Journal, Article ID, 365093: 10.

Dr. I.E. Cock et al.2015: GC-MS and LC-MS analysis of Kakadu plum fruit extracts displaying inhibitory activity against microbial triggers of multiple sclerosis, Pharmacognosy Communications Volume 5 | Issue 2

Gunnar Brandhorst and Michael Oellerich.2012: Liquid Chromatography–Tandem Mass Spectrometry or Automated Immunoassays: What Are the Future Trends in Therapeutic Drug Monitoring?, Clinical Chemistry 58(5): 821–825.

Steiner WE, English WA (2012) Emerging Trends in Liquid Chromatography and Mass Spectrometry Instrumentation for Analytical & Bio analytical Techniques. J Anal Bio anal Techniques 3:e106. doi:10.4172/2155-9872.1000e106

Wu AHB, French D.2012: Implementation of liquid chromatography/mass spectrometry into the clinical laboratory, Clin Chim Acta,

Thomas O metz, The future of liquid chromatography-mass spectrometry (LC-MS) in metabolic profiling and metabolomic studies for biomarker discovery, Biomark Med.1(1): 159–185.

Chi Chen, Frank J. Gonzalez & Jeffrey R. Idle.2007: LC-MS-Based Metabolomics in Drug Metabolism, Drug Metabolism Reviews, 39(2-3): 581-597.

Guodong Chen. 2009: Application of LC/MS to proteomics studies: current status and future prospects, Drug Discovery Today, 14(9–10) 465–471.

Sami Ahmed Khalid.2015: Pharmacovigilance of Herbal Remedies: Current State and Future Prospects, Conference: The 6th annual post-graduate conference, the medical & health Science studies, At Soba Medical Campus, Khartoum, Sudan.

Lee et al.2015, Organic–inorganic hybrid Nano flowers: types, characteristics, and future prospects, J Nano biotechnology, 13(54): 1-10.


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